For instance, competition for growth space and essential nutrients, and production of a wide range of antibiotics and enzymes counteract harmful microorganisms. Moreover production of siderophores also protect plants by solubilizing and scavenging iron from the environment, hence making it unavailable for other and more deleterious microorganisms. Phytohormones such as auxins, gibberellins, and cytokinins produced by certain bacteria stimulate growth of the plants. Many Bacillus ssp. have been found to provide beneficial effects to different plant species. The Gram-positive Bacillus group represents a large genetic and habitat diversity and has several properties of interest for applied use. Many Bacillus that can serve as biofertilizers and biopesticides are regarded non-pathogenic which makes handling easier. These bacteria are mobile and show good rhizosphere competence and are also facultative anaerobes enabling survival in soil at different environmental conditions. Bacillus sporulate under unfavorable conditions and the spores are very resistant to harsh conditions providing long shelf-life useful for commercial applications. Several Bacillus ssp. have high secretory capacity and certain strains are used as “cell factories” for industrial production of enzymes. Bacillus subtilis is one of the best studied bacteria out of many aspects impoving understanding of many processes and features. B. GSI-IX 208255-80-5 amyloliquefaciens subsp. plantarum strains are capable to enhance plant growth and confer protection by producing phytohormones and antimicrobial compounds. The type strain of the B. amyloliquefaciens subsp. plantarum group, FZB42, is known to have a great capacity for non-ribosomal synthesis of secondary metabolites including lipopeptides and polyketides by some gene clusters with antimicrobial and antifungal activity. Disease suppression by UCMB5113 has also been observed using Arabidopsis thaliana infected with fungal or bacterial pathogens. Further, improved tolerance of UCMB5113 treated wheat seedlings to abiotic stress factors like drought, cold and heat has been demonstrated. Thus, the UCMB5113 strain seems to have the capacity to operate on different plants, improve different kinds of stress management and stimulate plant growth making it an interesting candidate for use in agriculture to support more sustainable crop production. In this study, we describe the analysis of the B. amyloliquefaciens subsp. plantarum UCMB5113 genome sequence, and through comparison with the model species of the B. subtilis group, make an attempt to target genes.