Cell-based therapy in Benzethonium Chloride neurological diseases is an attractive option, but presents a difficult challenge the complex and precise interactions amongst them and the availability of appropriate cellular sources. Sources for cell Gentamycin Sulfate transplantation in the nervous system includes fetal neural tissues, embryonic stem cells, induced pluripotent stem cells, neural 
stem cells, non-neural somatic stem cells or even direct conversion of non-neural cells into neurons. Each of these cell types have the potential to replace cells lost to injury or disease or to modulate brain or spinal cord function; with each having their own advantages and disadvantages. Among the available options, NSCs are a promising choice as they retain the ability to generate a large number of cells from a relatively small amount of starting tissue and express the capacity for multi-lineage differentiation. However, NSC progeny are a heterogeneous cell population that exhibit poor survival and largely differentiate into glia following implantation into the mature CNS. In addition, a small population of the NSC progeny may retain a substantial proliferative potential. These caveats are further compounded by the poorly defined composition of cells within a multi-lineage NSC culture and the need for well characterized, highly purified cell phenotypes so as to reduce variability in pre-clinical and clinical investigations. To overcome these problems it is desirable to establish standard reproducible methodologies to generate highly enriched or relatively pure populations of cells. These cells can also be used for screening assays to uncover agents or niche-related conditions that enhance their survival, differentiation, neurite outgrowth and integration into the pre- existing circuitry of the adult CNS. With these aims in mind, and using cultured NSCs as a starting source of cells, here we show that using the distinct morphological characteristics of glial and neuronal cell populations, derived from differentiating NSC progeny, an enriched population of immature neurons can be isolated based solely on cell size and internal complexity. This enriched neuronal population contains a significant reduction in contaminating stem and progenitor cells, as evidenced by the in vitro neurosphere and neural colony forming cell assays. Screening a small panel of growth factors, we identified BMP-4 as a factor supporting the survival and maturation of the purified immature neuronal cells in vitro and following transplantation. Importantly, implanted cells retained their neuronal phenotype and showed no signs of excessive proliferative ability. Development of similar methodologies for purifying astrocytes and oligodendrocytes will provide the opportunity to deliver defined populations of cells into the CNS with the intent of enhancing donor integration and ultimately modifying host physiology. Resulting data were gated on bivariate displays, initially on forward and side scatter pulse area, to exclude debris and unwanted cells, and then on side scatter pulse width, versus side scatter pulse height to exclude doublets or cell clumps. Subsequent gates were set to exclude dead cells and select the cells, which represented the different cell populations of interest and/or showed staining above or below background. This suggests some degree of heterogeneity in the neuronal P1 population and that BMP4 does not have a survival effect on all GABAergic neurons derived from the Neurosphere Assay. Although immunocytochemistry suggested that the neurons would mature in culture, their utility for transplantation lay in their functional capabilities.