We conclude that GLA-AF is a potent emulsion-free adjuvant that warrants consideration for pandemic influenza vaccine development. Endovascular detachable coil treatment of intracranial aneurysms is an effective and increasingly popular non-invasive alternative to surgical clipping. In patients with ruptured intracranial aneurysms, endovascular intervention with detachable platinum coils can Forsythin improve the chances of independent survival compared with neurosurgical intervention to clip the neck of the aneurysm. The benefits of endovascular therapy for unruptured cerebral aneurysms have not been demonstrated, as the natural history of unruptured cerebral aneurysms has not been clearly defined. Nonetheless, when compared with surgical clipping, endovascular therapy of unruptured aneurysms is associated with decreased risk of adverse outcomes and in-hospital death, lower hospital charges, and 
shorter hospital stays. In future, the indications for and popularity of endovascular therapy is expected to increase further as better devices are developed. One of the substantial shortcomings of endovascular therapy is the technical problem of aneurysm recanalization. Recanalization due to coil compaction and aneurysmal regrowth is typically a consequence of the volume embolization rate at the time of treatment, choice of device material, localization of an aneurysmal remnant and hydromechanic factors. Recanalization should be avoided, as there is a high risk of aneurysmal rupture after endovascular therapy. There are a few reports of pathological evaluations of aneurysms after endovascular coil treatment. They suggest that a stable aneurysmal thrombosis is achieved if an endothelium-lined layer of connective tissue forms between the aneurysm and parent artery after embolization. Swine models are used as a basis for cerebral aneurysm Anemarsaponin-BIII research in many institutions, because aneurysm sizes are similar to those of humans and can be easily evaluated by angiography. The hemodynamic, physiologic, and histologic changes in experimental aneurysms in swine more closely reflect those observed humans than in other animal models. Furthermore, the swine model is useful for preclinical testing of new devices before research use in humans and ultimately in clinical practice. In particular, the swine model permits the effect of drugs and devices on the speed of endothelialization to be evaluated. There are many histopathological reports of aneurysm models, however, the time course of spontaneous thrombosis after Guglielmi detachable coil embolization in swine aneurysms has not been reported. We examined the expression profile of proliferating tissue covering the aneurysmal orifice using histochemical and morphological techniques in a swine model of sidewall-type cerebral aneurysm after coil embolization and spontaneous thrombosis. Recanalization of an embolized aneurysm is an independent longitudinal prognostic factor. Several studies have shown that stable aneurysmal thrombosis is associated with the formation of an endothelium-lined layer of connective tissue between the aneurysm and parent artery months after embolization. However, these findings do not show when and how early the layer of new endothelium develops over the neck of the obliterated.