We have recently constructed a DNA vaccine encoding the G glycoprotein from VHSV strain UK-860/94 and have demonstrated the high degree of protection provided against this virus as well as the production of specific neutralizing antibodies one month after vaccination. However, the early immune mechanisms implicated in the success of that vaccination remain still 28-demethyl-beta-amyrone unclear. Microarray technology is a very useful tool for the understanding of the immune process implicated in the protective response provided by efficient DNA vaccines against fish rhabdoviral infection. Some studies have been previously performed using microarrays, including the effect of a DNA vaccine encoding the infectious hematopoietic necrosis virus G glycoprotein in trout, the effect of the expression of the G protein from VHSV in Japanese flounder, as well as the differences in the gene expression profile following hirame rhabdovirus G and N protein DNA vaccination in Japanese flounder and the expression pattern after HIRRV challenge in vaccinated and non-vaccinated fish. However, the information provided by these reports was, in some cases, limited due to the relatively low number of annotated immune-related sequences included in the microarray. To our knowledge, this is the first global work in fish including both the analysis of the expression profile after DNA vaccination and the analysis of the differential transcriptomic patterns in vaccinated and non-vaccinated fish after rhabdoviral infection and the first performed in turbot. Moreover, the microarray has been constructed using a high number of annotated sequences obtained from an enriched 454-pyrosequencing of turbot transcriptome after viral stimulations, providing a higher quantity of information compared to previous similar publications in other fish species. The gene expression patterns of several immune-relevant pathways were Citiolone analyzed and allowed a better comprehension of the protective mechanisms underlying VHSV G protein DNA vaccination before and after VHSV challenge. Blast2GO suite was used for Gene Ontology classification into biological process terms of the significantly modulated genes from each comparison.