This is apparently due to two events that follow the receptor CC-115 engagement: i) the recruitment of CD95/Fas as well as other Tumor Necrosis Factor-family receptors to plasma membrane lipid rafts, and ii) the recruitment of specific proapoptotic bcl-2 family proteins to mitochondrial ����raft-like microdomains����. Indeed, small lipid domains are also present on mitochondrial membrane, where they may contribute to apoptosis-associated modifications of the organelle, i.e. its remodeling and fission, as well as to the release of apoptogenic factors and apoptosis execution. These raft-like microdomains are enriched in gangliosides and cardiolipin, but show a relatively low content of cholesterol; some molecules, including the voltage-dependent anion channel-1 and the fission protein hFis1, are enriched, whereas Bcl-2 family proteins are recruited, following CD95/Fas triggering. We showed the Homovanillic-acid association of GD3 with alpha and beta tubulin. In particular, in silico docking analysis showed that GD3 has a high affinity for the pore formed by four tubulin heterodimers, thus suggesting a possible interaction between tubulin and GD3. Hence, microtubules could act as tracks for ganglioside redistribution following apoptotic stimulation, possibly contributing to the mitochondrial alterations leading to cell death. The present work deals with the trafficking of glycosphingolipid GD3 to the mitochondrion upon pro-apoptotic triggering induced by CD95/Fas ligation and identifies the pivotal role of microtubule-associated protein CLIPR-59 in instructing and regulating GD3-microtubule association. The re-distribution of GD3 in lymphoblastoid T cells may play a decisive role in the apoptosis cascade. Previous works, including ours, identified the mitochondria as possible targets for GD3 and hypothesized that the rearrangement of GD3 may be involved in the mitochondrial remodeling leading to apoptosis execution phase. It was in fact suggested that mitochondria remodeling in terms of structural modifications, i.e. their curvature changes, as well as their fission process, could be under the influence of several molecules, including lipid microdomains.