The plasma concentration from oral administration was approximately 1 mg/ml, which quickly decreased. However, the metabolites of aniracetam remain much higher in the plasma concentration for many hours. There was some inconsistency with a previous study which administered 50 mg/kg of Aniracetam orally to rats and found that the half-life was 2.1 h in males and 1.7 h in females. Despite the inconsistent results the 50 mg/kg dose has been previously examined and does appear to be present in the plasma quickly after administration. Future studies could administer aniracetam and investigate learning and memory changes in tests such as novel object recognition and fear conditioning within 30 minutes of administration. Another Boldenone Undecylenate reason why we did not observe alterations in behavior with aniracetam treatment may be the dosage used in our study. In a previous study, the investigators presented the animals with 30 mg/kg and 100 mg/kg of oral administration of aniracetam. They found a significant increase in dopamine, serotonin, and their metabolites in the prefrontal cortex, dorsal hippocampus, and basolateral amygdala. In most regions the elevation lasted more than 180 minutes. One caveat of the study is that the elevation is seen in rats that are considered an animal model of multiple infarction. Since this is a disease model, it is not clear whether such changes occur in healthy rats. However, several studies have used a 50 mg/kg dose and found that this dose reverses learning and memory deficits and oral 50 mg/kg dose of aniracetam reverses impaired AMPA receptor mediated transmission in the hippocampus of rats prenatally exposed to ethanol. It is possible that a higher dose is necessary to result in improvement in learning and memory in normal healthy mice. However, we wanted to use a dose that has repeatedly been demonstrated to be effective in some experimental Maytansinol models. One study found that oral administration of 25 mg/kg and 100 mg/kg of aniracetam did not restore object recognition in 16- to 18month old rats while 50 mg/kg did restore object recognition.