PLCL was made of hydrophobic polymer and was not specially treated to increase hydrophilicity. Therefore it showed worse integration with ureter than natural scaffold. Acellular aortic arch scaffold because of its natural origin indicates very good integration with native ureter but other properties, especially scaffold diameter, caused conduit occlusion at the end-to-side anastomosis. Aortic arch have the largest diameter in rat, that is why we choose this vascular graft for experiment. Both scaffold types used in this experiment were disinfected before implantation using PBS with antibiotic, as previously described. Conditioned media obtained from both tested scaffolds were nontoxic after 24 and 48 h incubation with smooth muscle cell line. Slight cytotoxic effect was observed only after 72 h using PLCL conditioned medium, but cell growth analysis together with PLCL degradation test showed that cells growth well on PLCL surface starting to penetrate inside the scaffold. We used AA26-9 nonabsorbable sutures which served as a markers in this experiment. Despite use of such sutures and short follow-up, lack of stone formation inside the ureters and bladders were observed. Other important aspect was stoma formation in Group 1. Flat stoma can be result of improper urine collection in the bag and possible problem with the bag sticking to skin. To prevent the urine leakage we performed a stoma in the form of ����chimney���� because the diameter was too small to form nipple stoma in rat. Until 2012 only one paper about urinary conduit construction using tissue Canagliflozin hemihydrate engineering methods was available. In this work Drewa used SIS seeded and unseeded with 3T3 fibroblast cell line for urinary conduit creation. In three cases conduits were patent, seeding with 3T3 cells did not improve the results obtained, in this group inflammation process was more severe than in group with unseeded scaffold. In recent years another group described their attempts to make artificial urinary conduit using tissue engineering.Geutjes et al. used scaffold built from collagen type I and VyproII synthetic mesh for urinary conduit construction in 10 female pigs, but no differences between seeded and unseeded scaffolds were observed.